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Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity
Includes bibliographical references (leaves 161-184).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Division of Virology
2014
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| _version_ | 1867613294547697664 |
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| access_status_str | Open Access |
| author | Bere, Alfred |
| author2 | Passmore, Jo-Ann |
| author_browse | Bere, Alfred Passmore, Jo-Ann |
| author_facet | Passmore, Jo-Ann Bere, Alfred |
| author_sort | Bere, Alfred |
| collection | Thesis |
| description | Includes bibliographical references (leaves 161-184). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10081 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:51.607Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Virology |
| publisherStr | Division of Virology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10081 Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity Bere, Alfred Passmore, Jo-Ann Medical Virology Includes bibliographical references (leaves 161-184). This study shows that HIV-specific cervical T cells can be isolated by cytobrushing and in vitro polyclonal expansion is a useful approach to increase the number of T cells available from mucosal sites. Dynal beads (1:1) in the presence of IL-2, IL-7 and IL-15 resulted in the best yields of cervical T cells while anti-CD3 in the presence of IL-2 best conserved the ex vivo T cell profile. Expanded T cell lines, irrespective of expansion method used, generally maintain their cytokine response profile to HIV anti- gens. This study shows that HIV Gag-specific blood and cervical T cells were largely mono-functional with polyfunctional T cells being detected in women with high blood CD4 count and low plasma viral load. This study confirms that HIV-specific Gag T cell responses detected in the polyclonal expanded female genital tract T cells are associated with those measured in blood during HIV infection. 2014-12-26T14:01:36Z 2014-12-26T14:01:36Z 2010 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/10081 eng application/pdf Division of Virology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Virology Bere, Alfred Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| thesis_degree_str | Doctoral |
| title | Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| title_full | Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| title_fullStr | Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| title_full_unstemmed | Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| title_short | Comparison of HIV-1 specific T cell immunity in the female genital tract and blood of HIV-infected women : impact of in vitro T cell expansion on HIV-specific T cell specificity, maturational status and functional complexity |
| title_sort | comparison of hiv 1 specific t cell immunity in the female genital tract and blood of hiv infected women impact of in vitro t cell expansion on hiv specific t cell specificity maturational status and functional complexity |
| topic | Medical Virology |
| url | http://hdl.handle.net/11427/10081 |
| work_keys_str_mv | AT berealfred comparisonofhiv1specifictcellimmunityinthefemalegenitaltractandbloodofhivinfectedwomenimpactofinvitrotcellexpansiononhivspecifictcellspecificitymaturationalstatusandfunctionalcomplexity |