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Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris
Includes bibliographical references (leaves 98-103).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Centre for Bioprocess Engineering Research
2014
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| _version_ | 1867613254787792896 |
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| access_status_str | Open Access |
| author | Bhardwaj, Vinayak |
| author2 | Harrison, STL |
| author_browse | Bhardwaj, Vinayak Harrison, STL |
| author_facet | Harrison, STL Bhardwaj, Vinayak |
| author_sort | Bhardwaj, Vinayak |
| collection | Thesis |
| description | Includes bibliographical references (leaves 98-103). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10120 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:13.838Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Centre for Bioprocess Engineering Research |
| publisherStr | Centre for Bioprocess Engineering Research |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10120 Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris Bhardwaj, Vinayak Harrison, STL Minnaar, Sanet Bioprocess Engineering Includes bibliographical references (leaves 98-103). The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics. For this purpose, the methylotrophic yeast Pichia pastoris has emerged as an additional microbial host for recombinant insulin production. A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding strategy in which growth was optimised by feeding glycerol to boost biomass followed by induction of the gene encoding insulin precursor by feeding methanol. 2014-12-26T14:15:27Z 2014-12-26T14:15:27Z 2011 Master Thesis Masters MSc http://hdl.handle.net/11427/10120 eng application/pdf Centre for Bioprocess Engineering Research Faculty of Engineering and the Built Environment University of Cape Town |
| spellingShingle | Bioprocess Engineering Bhardwaj, Vinayak Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| thesis_degree_str | Master's |
| title | Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| title_full | Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| title_fullStr | Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| title_full_unstemmed | Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| title_short | Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris |
| title_sort | design of an optimised fed batch process for insulin precursor production in pichia pastoris |
| topic | Bioprocess Engineering |
| url | http://hdl.handle.net/11427/10120 |
| work_keys_str_mv | AT bhardwajvinayak designofanoptimisedfedbatchprocessforinsulinprecursorproductioninpichiapastoris |