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Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis
Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613208746917888 |
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| access_status_str | Open Access |
| author | Ginbot, Zekarias Gebremedhin |
| author2 | Farrant, Jill M |
| author_browse | Farrant, Jill M Ginbot, Zekarias Gebremedhin |
| author_facet | Farrant, Jill M Ginbot, Zekarias Gebremedhin |
| author_sort | Ginbot, Zekarias Gebremedhin |
| collection | Thesis |
| description | Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10523 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:29.432Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10523 Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis Ginbot, Zekarias Gebremedhin Farrant, Jill M Illing, Nicola Cell Biology Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies were generated and used in determination of expression conditions and immunolocalization studies. 2014-12-30T06:42:31Z 2014-12-30T06:42:31Z 2011 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/10523 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Ginbot, Zekarias Gebremedhin Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| thesis_degree_str | Doctoral |
| title | Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| title_full | Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| title_fullStr | Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| title_full_unstemmed | Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| title_short | Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis |
| title_sort | characterization of two desiccation linked group 1 lea proteins from the resurrection plant xerophyta humilis |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/10523 |
| work_keys_str_mv | AT ginbotzekariasgebremedhin characterizationoftwodesiccationlinkedgroup1leaproteinsfromtheresurrectionplantxerophytahumilis |