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Process integration in the optimisation of amidase production from recombinant Escherichia coli
Includes abstract.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Centre for Bioprocess Engineering Research
2015
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| _version_ | 1867613638440779776 |
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| access_status_str | Open Access |
| author | Olaofe, Oluwafemi Ayokunle |
| author2 | Harrison, STL |
| author_browse | Harrison, STL Olaofe, Oluwafemi Ayokunle |
| author_facet | Harrison, STL Olaofe, Oluwafemi Ayokunle |
| author_sort | Olaofe, Oluwafemi Ayokunle |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/10857 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:39:19.951Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Centre for Bioprocess Engineering Research |
| publisherStr | Centre for Bioprocess Engineering Research |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/10857 Process integration in the optimisation of amidase production from recombinant Escherichia coli Olaofe, Oluwafemi Ayokunle Harrison, STL Bioprocess Engineering Includes abstract. Includes bibliographical references (p. 127-138). This thesis presents the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit operations in the amidase purification train. The EBA process can integrate up to four steps by merging processes involved in centrifugation, microfiltration and initial adsorption into one unit operation. 2015-01-01T12:42:15Z 2015-01-01T12:42:15Z 2008 Master Thesis Masters MSc http://hdl.handle.net/11427/10857 eng application/pdf Centre for Bioprocess Engineering Research Faculty of Engineering and the Built Environment University of Cape Town |
| spellingShingle | Bioprocess Engineering Olaofe, Oluwafemi Ayokunle Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| thesis_degree_str | Master's |
| title | Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| title_full | Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| title_fullStr | Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| title_full_unstemmed | Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| title_short | Process integration in the optimisation of amidase production from recombinant Escherichia coli |
| title_sort | process integration in the optimisation of amidase production from recombinant escherichia coli |
| topic | Bioprocess Engineering |
| url | http://hdl.handle.net/11427/10857 |
| work_keys_str_mv | AT olaofeoluwafemiayokunle processintegrationintheoptimisationofamidaseproductionfromrecombinantescherichiacoli |