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Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection
The aim of this project was to compare the breadth and magnitude of CFP10 and ESAT6-specific CD8 T cell responses in individuals with latent Mycobacterium tuberculosis (MTB) infection (LTBI) and active TB disease, and further define MTB-specific CD8 T cell phenotypes associated with latent infection...
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| Format: | Thesis |
| Language: | English |
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Division of Immunology
2015
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| _version_ | 1867613462563127297 |
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| access_status_str | Open Access |
| author | Moshi, Noell Dominika |
| author2 | Day, Cheryl |
| author_browse | Day, Cheryl Moshi, Noell Dominika |
| author_facet | Day, Cheryl Moshi, Noell Dominika |
| author_sort | Moshi, Noell Dominika |
| collection | Thesis |
| description | The aim of this project was to compare the breadth and magnitude of CFP10 and ESAT6-specific CD8 T cell responses in individuals with latent Mycobacterium tuberculosis (MTB) infection (LTBI) and active TB disease, and further define MTB-specific CD8 T cell phenotypes associated with latent infection and active disease. Ex vivo IFN? Elispots and proliferation assays were used to identify immunodominant ESAT6 and CFP10 15mer peptides targeted by CD8 T cells in LTBI and TB donors. A multiparameter flow cytometry panel was designed and optimized to assess turnover, susceptibility to apoptosis and terminal differentiation/senescence in CD8 T cells from TB and LTBI donors. Bcl-2, Ki67,CD95, CD57, CD127 and IFNγ were thus measured in each group. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/11785 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:36:32.221Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Division of Immunology |
| publisherStr | Division of Immunology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/11785 Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection Moshi, Noell Dominika Day, Cheryl Hanekom, Willem A Clinical Science and Immunology The aim of this project was to compare the breadth and magnitude of CFP10 and ESAT6-specific CD8 T cell responses in individuals with latent Mycobacterium tuberculosis (MTB) infection (LTBI) and active TB disease, and further define MTB-specific CD8 T cell phenotypes associated with latent infection and active disease. Ex vivo IFN? Elispots and proliferation assays were used to identify immunodominant ESAT6 and CFP10 15mer peptides targeted by CD8 T cells in LTBI and TB donors. A multiparameter flow cytometry panel was designed and optimized to assess turnover, susceptibility to apoptosis and terminal differentiation/senescence in CD8 T cells from TB and LTBI donors. Bcl-2, Ki67,CD95, CD57, CD127 and IFNγ were thus measured in each group. 2015-01-08T19:59:34Z 2015-01-08T19:59:34Z 2011 Master Thesis Masters MSc http://hdl.handle.net/11427/11785 eng application/pdf Division of Immunology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Clinical Science and Immunology Moshi, Noell Dominika Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| thesis_degree_str | Master's |
| title | Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| title_full | Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| title_fullStr | Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| title_full_unstemmed | Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| title_short | Characterization of CD8 T cell responses in Mycobacterium Tuberculosis infection |
| title_sort | characterization of cd8 t cell responses in mycobacterium tuberculosis infection |
| topic | Clinical Science and Immunology |
| url | http://hdl.handle.net/11427/11785 |
| work_keys_str_mv | AT moshinoelldominika characterizationofcd8tcellresponsesinmycobacteriumtuberculosisinfection |