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Beak and feather disease virus candidate vaccine development
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2015
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| _version_ | 1867613342278877184 |
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| access_status_str | Open Access |
| author | Duvenage, Lucian |
| author2 | Rybicki, Ed |
| author_browse | Duvenage, Lucian Rybicki, Ed |
| author_facet | Rybicki, Ed Duvenage, Lucian |
| author_sort | Duvenage, Lucian |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/12785 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:36.552Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/12785 Beak and feather disease virus candidate vaccine development Duvenage, Lucian Rybicki, Ed Hitzeroth, Inga Meyers, Ann Molecular and Cell Biology Includes bibliographical references. [Fix supervisors field.] Psittacine beak and feather disease, caused by a circovirus known as beak and feather disease virus (BFDV), is a threat to both wild and captive psittacine species. There is currently no vaccine against BFDV and safe and affordable vaccine candidates are needed to alleviate the disease burden caused by this virus. Production of the BFDV's major antigenic determinant, the capsid protein (CP), in the inexpensive and highly scalable plant expression system, could satisfy these requirements as a potential subunit vaccine. In this work, truncated CP (ÄN40 CP) was first expressed in E. coli to successfully generate anti-CP polyclonal antibodies. ÄN40 CP and full-length CP transient expression in tobacco (Nicotiana benthamiana) was optimised as fusions to elastin-like polypeptide (ELP). Fusion of CP or ÄN40 CP to ELPs of different lengths was shown to increase yield relative to unfused CP/ÄN40 CP. Free ELP and a GFP-ELP fusion could be purified by inverse transition cycling (ITC), using centrifugation and membrane filtration methods. A ÄN40 CP-ELP fusion expressed in plants could be partially purified and represents low-cost vaccine candidate against BFDV. A candidate DNA vaccine expressing ÄN40 CP was also evaluated for expression of the antigen in vitro and may prove useful in a prime-boost regimen together with one of the plant-produced vaccine candidates. 2015-05-13T14:15:55Z 2015-05-13T14:15:55Z 2012 Master Thesis Masters MSc http://hdl.handle.net/11427/12785 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Duvenage, Lucian Beak and feather disease virus candidate vaccine development |
| thesis_degree_str | Master's |
| title | Beak and feather disease virus candidate vaccine development |
| title_full | Beak and feather disease virus candidate vaccine development |
| title_fullStr | Beak and feather disease virus candidate vaccine development |
| title_full_unstemmed | Beak and feather disease virus candidate vaccine development |
| title_short | Beak and feather disease virus candidate vaccine development |
| title_sort | beak and feather disease virus candidate vaccine development |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/12785 |
| work_keys_str_mv | AT duvenagelucian beakandfeatherdiseaseviruscandidatevaccinedevelopment |