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Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system
Bibliography: p. 86-109.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613226209902593 |
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| access_status_str | Open Access |
| author | Mills, Martin George |
| author2 | Klump, Horst |
| author_browse | Klump, Horst Mills, Martin George |
| author_facet | Klump, Horst Mills, Martin George |
| author_sort | Mills, Martin George |
| collection | Thesis |
| description | Bibliography: p. 86-109. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/17946 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:46.693Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/17946 Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system Mills, Martin George Klump, Horst Biochemistry Bibliography: p. 86-109. Triple helices are classified into two groups according to the composition and orientation of the third strand, namely the pyrimidine motif and the purine motif. These motifs constitute two separate fields of research. It was proposed, based on the alternative design rules, that the two motifs can in fact lead to competing structures. An oligonucleotide system has been designed to demonstrate this competition. Systematic variation of its components give insight into the requirements for optimal binding of a third strand. A palindromic, homopyrimidine oligonucleotide of 22 bases was designed to form an overlapping 9-base, Watson-Crick (WC), duplex with a partly complementary 22-base purine-rich oligonucleotide. This leaves two free 3' extensions under conditions when only the duplex is stable. If the conditions, however, favour triplex formation the pyrimidine tail can compete with the purine-rich tail as third strand for the duplex forming Hoogsteen or reverse-Hoogsteen hydrogen bonding respectively with the purines in the WC double strand. The underlying triplexes and core duplex were synthesised and characterised as controls. 2016-03-17T12:41:06Z 2016-03-17T12:41:06Z 1998 Master Thesis Masters MSc http://hdl.handle.net/11427/17946 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Biochemistry Mills, Martin George Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| thesis_degree_str | Master's |
| title | Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| title_full | Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| title_fullStr | Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| title_full_unstemmed | Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| title_short | Competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| title_sort | competition between the purine and pyrimidine triple helix motifs in an oligonucleotide system |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/17946 |
| work_keys_str_mv | AT millsmartingeorge competitionbetweenthepurineandpyrimidinetriplehelixmotifsinanoligonucleotidesystem |