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A study of the molecular variability of some South African isolates of tobacco necrosis virus
Bibliography: pages 109-118.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613289215688704 |
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| access_status_str | Open Access |
| author | Freeborough, Michael-John |
| author2 | Von Wechmar, M Barbara |
| author_browse | Freeborough, Michael-John Von Wechmar, M Barbara |
| author_facet | Von Wechmar, M Barbara Freeborough, Michael-John |
| author_sort | Freeborough, Michael-John |
| collection | Thesis |
| description | Bibliography: pages 109-118. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/20192 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:45.686Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/20192 A study of the molecular variability of some South African isolates of tobacco necrosis virus Freeborough, Michael-John Von Wechmar, M Barbara Purves, M Molecular and Cell Biology Bibliography: pages 109-118. The relationship of various tobacco necrosis viruses, isolated from various crops and other sources in South Africa was determined. An isolate from avocado was chosen for partial characterisation to confirm that it was a member of the Necrovirus group of plant viruses. TNV was detected in potatoes that exhibited abnormal necrotic foliar symptoms and a D-type TNV was isolated and identified from the Up-to-Date potato variety. Immunoelectroblotting assay grouped the TNV isolates studied into two serotypes (A and D). This result was confirmed by NA hybridization with probes derived to the coat protein of an A- (TNVWheat) and a D-type (TNV-Papaya green lesion) isolate. RT-PCR with A and D specific primers did not amplify the coat protein of three A- and D-type TNV isolates which appears to indicate that the detection by PCR with specific primers is too selective to be used for a general test, unless degenerate primers to a more conserved region of the coat protein gene are used. Sequence analysis of the coat protein gene was used to determine the phylogenetic relationship amongst nine TNV isolates examined and also by comparison to three isolates for which the coat protein gene had already been sequenced. Sequence analysis showed high degree of homology amongst the A-type isolates and the D-type isolates, with approximately 45 % homology between the two TNV types. 2016-07-04T08:42:26Z 2016-07-04T08:42:26Z 1996 Master Thesis Masters MSc http://hdl.handle.net/11427/20192 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Freeborough, Michael-John A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| thesis_degree_str | Master's |
| title | A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| title_full | A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| title_fullStr | A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| title_full_unstemmed | A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| title_short | A study of the molecular variability of some South African isolates of tobacco necrosis virus |
| title_sort | study of the molecular variability of some south african isolates of tobacco necrosis virus |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/20192 |
| work_keys_str_mv | AT freeboroughmichaeljohn astudyofthemolecularvariabilityofsomesouthafricanisolatesoftobacconecrosisvirus AT freeboroughmichaeljohn studyofthemolecularvariabilityofsomesouthafricanisolatesoftobacconecrosisvirus |