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The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains
Bibliography: pages 199-213.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613293392166912 |
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| access_status_str | Open Access |
| author | Schnippenkoetter, Wendelin Heribert |
| author2 | Rybicki, Edward P |
| author_browse | Rybicki, Edward P Schnippenkoetter, Wendelin Heribert |
| author_facet | Rybicki, Edward P Schnippenkoetter, Wendelin Heribert |
| author_sort | Schnippenkoetter, Wendelin Heribert |
| collection | Thesis |
| description | Bibliography: pages 199-213. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21412 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:49.949Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21412 The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains Schnippenkoetter, Wendelin Heribert Rybicki, Edward P Molecular and Cell Biology Bibliography: pages 199-213. The infectivity of the replicative form (RF) DNAs of MSV-Kom, MSV-Set and PanSV-Kar contained in the plasm ids pKom500, pSet100 and pPS100 was established by agroinoculating susceptible Jubilee sweetcorn with partial homodimeric Agrobacterium tumefaciens (C58C1) clones of RF-DNAs. Biological characteristics typical of Mastreviruses; such as, the appearance and leafhopper transmissibility of streak symptoms on infected plants, the presence of 18x30nm geminate particles in electron micrographs of leaf-dip preparations, and the presence of single-stranded and double-stranded DNA in Southern blot tests of infected plant DNA extracts, indicated that the RF-DNAs in pKom500, pSet100and PanSV-Kar represent the entire genomes of MSV-Kom, MSV-Set and PanSV-Karrespectively. The complete nucleotide (nt) sequence of the genome of MSV-Set was determined and characterised, and compared with those of MSV-Kom and PanSV-Kar. The genome sizes of MSV-Kom, MSV-Set and PanSV-Kar are 2701, 2690 and 2705 nt respectively, and all share Mastreviral genomic features. Phylogenetic analyses on the nt sequences and the putative amino acid sequences of the movement, coat and replication-associated proteins (MP, CP and Rep respectively) indicate that MSV-Set is grouped with, yet distinct from the MSV group of viruses isolated from maize. MSV-Set shares a 78% nt sequence identity with MSV-Kom which shares a >96% nt sequence identity with other MSVs. The PanSV-Kar genome shares a 60% nt sequence identity with the MSV group and89% with the Kenyan PanSV-Ken. PanSV-Kar causes mild non-persistent streak in Jubilee sweetcorn. MSV-Kom (previously isolated from maize in Komatipoort, Mpumalanga) and MSV-Set (previously isolated from a Setaria species in Mt. Edgecombe, Kwazulu/Natal) have different pathogenicities, and have overlapping, but non-identical, host ranges. Leafhopper transmission tests determined that MSV-Kom and MSV-Set generally cause severe and moderate streak in maize cultivars, or mild and severe streak in wheat cultivars respectively. 2016-08-22T12:22:09Z 2016-08-22T12:22:09Z 1998 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21412 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and Cell Biology Schnippenkoetter, Wendelin Heribert The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| thesis_degree_str | Doctoral |
| title | The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| title_full | The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| title_fullStr | The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| title_full_unstemmed | The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| title_short | The use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| title_sort | use of agroinfectious clones to investigate recombination between distinct maize streak virus strains |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/21412 |
| work_keys_str_mv | AT schnippenkoetterwendelinheribert theuseofagroinfectiousclonestoinvestigaterecombinationbetweendistinctmaizestreakvirusstrains AT schnippenkoetterwendelinheribert useofagroinfectiousclonestoinvestigaterecombinationbetweendistinctmaizestreakvirusstrains |