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Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger
The aim of this project is to establish and understand the production of glucose oxidase and gluconic acid using the Aspergillus niger bioprocess and predict its response to operating conditions. Glucose oxidase and gluconic acid are produced by a wide range of microbes and have a variety of applica...
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| Format: | Thesis |
| Language: | English |
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Centre for Bioprocess Engineering Research
2016
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| _version_ | 1867613337465913344 |
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| access_status_str | Open Access |
| author | Johnson, Kim Henry Silvanus |
| author2 | Harrison, STL |
| author_browse | Harrison, STL Johnson, Kim Henry Silvanus |
| author_facet | Harrison, STL Johnson, Kim Henry Silvanus |
| author_sort | Johnson, Kim Henry Silvanus |
| collection | Thesis |
| description | The aim of this project is to establish and understand the production of glucose oxidase and gluconic acid using the Aspergillus niger bioprocess and predict its response to operating conditions. Glucose oxidase and gluconic acid are produced by a wide range of microbes and have a variety of applications. In this study Aspergillus niger was chosen as the microorganism as it has the "generally accepted as safe" (gras) status in the U.S.A. It is also the major industrial producer. Glucose oxidase catalyses the conversion of glucose, oxygen and water to hydrogen peroxide and gluconic acid. This enzyme is used as a glucose and oxygen scavenger in the food industry and as a diagnostic tool in medicine for glucose determination. Gluconic acid is an organic acid used as a sequestering agent with a broad spectrum of applications. The world market for gluconic acid and its various salts was 45 000 metric tonnes in 1985 (Bigelis cited by Markwell et al. 1989). Gluconic acid and its derivatives can be produced using three technologies: electrolysis, mild chemical oxidation and bioprocess. The first two technologies have not been proven to be comnercially viable. The bioprocess offers diversity of feed and produces other products such as glucose oxidase. Literature has shown that the production of gluconic acid involves two kinetic areas. Firstly, the glucose oxidase enzyme must be induced. Secondly, glucose is converted to gluconic acid by the enzyme glucose oxidase. The factors affecting the kinetics associated with the induction of glucose oxidase have only been described qualitatively. Glucose, oxygen and pH have been shown to affect the induction of glucose oxidase. The effect of pH has been studied by Roukas and Harvey (1989) who found that induction, is maximal at a pH of between 5 and 6. The effect of glucose and oxygen have not been quantified. The kinetics of glucose oxidase conversion of glucose to gluconic acid have been well described by Atkinson and Lester (1974). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21517 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:32.198Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Centre for Bioprocess Engineering Research |
| publisherStr | Centre for Bioprocess Engineering Research |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21517 Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger Johnson, Kim Henry Silvanus Harrison, STL Bioprocess Engineering The aim of this project is to establish and understand the production of glucose oxidase and gluconic acid using the Aspergillus niger bioprocess and predict its response to operating conditions. Glucose oxidase and gluconic acid are produced by a wide range of microbes and have a variety of applications. In this study Aspergillus niger was chosen as the microorganism as it has the "generally accepted as safe" (gras) status in the U.S.A. It is also the major industrial producer. Glucose oxidase catalyses the conversion of glucose, oxygen and water to hydrogen peroxide and gluconic acid. This enzyme is used as a glucose and oxygen scavenger in the food industry and as a diagnostic tool in medicine for glucose determination. Gluconic acid is an organic acid used as a sequestering agent with a broad spectrum of applications. The world market for gluconic acid and its various salts was 45 000 metric tonnes in 1985 (Bigelis cited by Markwell et al. 1989). Gluconic acid and its derivatives can be produced using three technologies: electrolysis, mild chemical oxidation and bioprocess. The first two technologies have not been proven to be comnercially viable. The bioprocess offers diversity of feed and produces other products such as glucose oxidase. Literature has shown that the production of gluconic acid involves two kinetic areas. Firstly, the glucose oxidase enzyme must be induced. Secondly, glucose is converted to gluconic acid by the enzyme glucose oxidase. The factors affecting the kinetics associated with the induction of glucose oxidase have only been described qualitatively. Glucose, oxygen and pH have been shown to affect the induction of glucose oxidase. The effect of pH has been studied by Roukas and Harvey (1989) who found that induction, is maximal at a pH of between 5 and 6. The effect of glucose and oxygen have not been quantified. The kinetics of glucose oxidase conversion of glucose to gluconic acid have been well described by Atkinson and Lester (1974). 2016-08-24T13:00:20Z 2016-08-24T13:00:20Z 1995 Master Thesis Masters MSc (Eng) http://hdl.handle.net/11427/21517 eng application/pdf Centre for Bioprocess Engineering Research Faculty of Engineering and the Built Environment University of Cape Town |
| spellingShingle | Bioprocess Engineering Johnson, Kim Henry Silvanus Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| thesis_degree_str | Master's |
| title | Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| title_full | Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| title_fullStr | Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| title_full_unstemmed | Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| title_short | Glucose oxidase induction and the modelling of gluconic acid production using Aspergillus niger |
| title_sort | glucose oxidase induction and the modelling of gluconic acid production using aspergillus niger |
| topic | Bioprocess Engineering |
| url | http://hdl.handle.net/11427/21517 |
| work_keys_str_mv | AT johnsonkimhenrysilvanus glucoseoxidaseinductionandthemodellingofgluconicacidproductionusingaspergillusniger |