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Molecular investigations of subgroup I geminiviruses
Bibliography: pages 139-150.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867614432249511936 |
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| access_status_str | Open Access |
| author | Hughes, Fiona Lesley |
| author2 | Rybicki, Edward P |
| author_browse | Hughes, Fiona Lesley Rybicki, Edward P |
| author_facet | Rybicki, Edward P Hughes, Fiona Lesley |
| author_sort | Hughes, Fiona Lesley |
| collection | Thesis |
| description | Bibliography: pages 139-150. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21979 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:51:56.986Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21979 Molecular investigations of subgroup I geminiviruses Hughes, Fiona Lesley Rybicki, Edward P Virology Bibliography: pages 139-150. The diversity of Subgroup I geminiviruses causing streak disease in maize, sugarcane, and indigenous wild grasses was investigated. The virus. isolates studied originated from maize (several southern African isolates), two sugarcane cultivars (from Natal province, South Africa, and from Mauritius), wheat, and three grasses (Panicum, Setaria, and Eleusine spp. from South Africa). The following methods were used: analysis of restriction fragment length polymorphisms (RFLPs) between viral genomes in individual infected plants; DNA cross-hybridization between virus isolates; restriction endonuclease mapping of whole virus genomes; and nucleic acid sequencing. The complete genome of the Natal sugarcane streak virus isolate was sequenced. Partial sequences were obtained for other isolates, either by sequencing the ends of cloned viral genomes, or by sequencing a 250 base pair fragment of a highly conserved open reading frame that had been amplified using the polymerase chain reaction technique. The viruses being studied were compared both among themselves and with other Subgroup I geminiviruses of known DNA sequence, on the basis of sequence (nucleotide and amino acid) and restriction map data. Distance matrix methods were used to infer phylogenetic relationships between Subgroup I geminiviruses from restriction map and sequence data. Phylogenies deduced from sequence data were considered to be more accurate than those deduced from map data. Regardless of the method of analysis used, however, the relationships between the Subgroup I geminiviruses studied here remained constant. Thus, three strains of MSV (maize, Setaria, and Eleusine strains) were distinguished. Streak viruses distinct from MSV were also identified: panicum streak virus (PanSV), and two distantly related strains (Natal and Mauritius) of sugarcane streak virus (SSV). Restriction mapping of different geographical isolates of the maize strain of MSV demonstrated that variation existed within a single strain of virus. RFLP analysis indicated that minor variation existed between virus genomes within single diseased plants. Methods used to. type Subgroup I geminiviruses were evaluated, and discrepancies in the serological typing of geminiviruses from Subgroups I and III were pointed out. A unified scheme was proposed for distinguishing between distinct Subgroup I geminiviruses and strains of geminiviruses. The origins of maize and sugarcane streak viruses were speculated upon. 2016-09-28T19:05:18Z 2016-09-28T19:05:18Z 1991 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21979 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Virology Hughes, Fiona Lesley Molecular investigations of subgroup I geminiviruses |
| thesis_degree_str | Doctoral |
| title | Molecular investigations of subgroup I geminiviruses |
| title_full | Molecular investigations of subgroup I geminiviruses |
| title_fullStr | Molecular investigations of subgroup I geminiviruses |
| title_full_unstemmed | Molecular investigations of subgroup I geminiviruses |
| title_short | Molecular investigations of subgroup I geminiviruses |
| title_sort | molecular investigations of subgroup i geminiviruses |
| topic | Virology |
| url | http://hdl.handle.net/11427/21979 |
| work_keys_str_mv | AT hughesfionalesley molecularinvestigationsofsubgroupigeminiviruses |