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The isolation and characterization of a P. Angulosus homeobox
Bibliography: pages 93-108.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2016
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| _version_ | 1867613163718967296 |
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| access_status_str | Open Access |
| author | Pfeffer, Peter Lance |
| author2 | Von Holt, Claus |
| author_browse | Pfeffer, Peter Lance Von Holt, Claus |
| author_facet | Von Holt, Claus Pfeffer, Peter Lance |
| author_sort | Pfeffer, Peter Lance |
| collection | Thesis |
| description | Bibliography: pages 93-108. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/21983 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:47.142Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/21983 The isolation and characterization of a P. Angulosus homeobox Pfeffer, Peter Lance Von Holt, Claus Biochemistry Bibliography: pages 93-108. The aim of this thesis was to isolate and characterize a homeobox-containing gene of the South African sea urchin Parechinus angulosus. This was achieved by constructing a genomic library of several individuals and screening this library using a probe containing the Antennapedia homeobox. Eight clones were isolated and shown to represent different alleles of the same gene. One clone was sequenced, revealing a homeobox which was termed PaHboxl. This homeobox was compared to published homeobox sequences and shown to be a member of the Antp (Hoxl.l) subclass (table 1.1). A splice donor site was identified 23 bp upstream of the homeobox and the observation confirmed by RNAase mapping. PaHboxl is situated in a genomic area showing a significantly higher degree of restriction fragment polymorphism than expected. This was shown by a statistical analysis which should be of general value in the interpretation of such polymorphisms. The expression of PaHboxl was examined by RNAase protection assays and Northern blotting. Two distinct phases of expression were observed - during embryogenesis PaHboxl is expressed transiently at low levels in 11,5 hr mesenchyme blastula stage embryos (44 ± 8 transcripts per embryo) with levels 3-5 fold lower 2,5 hr before and after this stage. Expression is observed again at up to 160 fold higher levels in the adult with maximal expression in testis (11 transcripts per 10 pg total RNA), and increasingly lower levels in intestines, ovary and Aristotle's lantern. Two transcripts of size 5,2 and 5,7 kbp were observed. Expression in Aristotle's lantern and embryonic stages could not be detected by Northern analysis. 2016-09-28T19:05:27Z 2016-09-28T19:05:27Z 1991 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/21983 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Biochemistry Pfeffer, Peter Lance The isolation and characterization of a P. Angulosus homeobox |
| thesis_degree_str | Doctoral |
| title | The isolation and characterization of a P. Angulosus homeobox |
| title_full | The isolation and characterization of a P. Angulosus homeobox |
| title_fullStr | The isolation and characterization of a P. Angulosus homeobox |
| title_full_unstemmed | The isolation and characterization of a P. Angulosus homeobox |
| title_short | The isolation and characterization of a P. Angulosus homeobox |
| title_sort | isolation and characterization of a p angulosus homeobox |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/21983 |
| work_keys_str_mv | AT pfefferpeterlance theisolationandcharacterizationofapangulosushomeobox AT pfefferpeterlance isolationandcharacterizationofapangulosushomeobox |