Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262
The aim of this study was to characterise the relationships between solventogenesis and endospore formation in Clostridium acetobutylicum strain P262. Growth and endospore formation was monitored in a number of complex and minimal media and as a result of these studies a new defined sporulation medi...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English English |
| Published: |
Department of Molecular and Cell Biology
2017
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613328874930176 |
|---|---|
| access_status_str | Open Access |
| author | Long, Susan |
| author2 | Woods, D R |
| author_browse | Long, Susan Woods, D R |
| author_facet | Woods, D R Long, Susan |
| author_sort | Long, Susan |
| collection | Thesis |
| description | The aim of this study was to characterise the relationships between solventogenesis and endospore formation in Clostridium acetobutylicum strain P262. Growth and endospore formation was monitored in a number of complex and minimal media and as a result of these studies a new defined sporulation medium was developed. The defined system produced high levels of solvents and supported 60 80% sporulation in C. acetobutylicum P262. Endospore formation occurred near-synchronously, enabling this system to be used in correlative physiological and morphological studies. Five other Type Culture Clostridium strains grew and sporulated less well in the C. acetobutylicum minimal medium (CAMM). These variations emphasise the importance of strain differences amongst the Clostridia. Two well defined physiological phases, the acidogenic phase and the solventogenic phase, which characterise the industrial ABE fermentation process were observed in CAMM. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/23277 |
| institution | University of Cape Town (South Africa) |
| language | eng eng |
| last_indexed | 2026-06-10T12:34:23.309Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/23277 Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 Long, Susan Woods, D R Jones, D T Microbiology The aim of this study was to characterise the relationships between solventogenesis and endospore formation in Clostridium acetobutylicum strain P262. Growth and endospore formation was monitored in a number of complex and minimal media and as a result of these studies a new defined sporulation medium was developed. The defined system produced high levels of solvents and supported 60 80% sporulation in C. acetobutylicum P262. Endospore formation occurred near-synchronously, enabling this system to be used in correlative physiological and morphological studies. Five other Type Culture Clostridium strains grew and sporulated less well in the C. acetobutylicum minimal medium (CAMM). These variations emphasise the importance of strain differences amongst the Clostridia. Two well defined physiological phases, the acidogenic phase and the solventogenic phase, which characterise the industrial ABE fermentation process were observed in CAMM. 2017-01-26T07:52:36Z 2017-01-26T07:52:36Z 1984 2016-11-22T09:59:47Z Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/23277 eng eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Long, Susan Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| thesis_degree_str | Doctoral |
| title | Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| title_full | Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| title_fullStr | Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| title_full_unstemmed | Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| title_short | Studies on the regulation of solvent production and endospore formation in Clostridium Acetobutylicum P262 |
| title_sort | studies on the regulation of solvent production and endospore formation in clostridium acetobutylicum p262 |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/23277 |
| work_keys_str_mv | AT longsusan studiesontheregulationofsolventproductionandendosporeformationinclostridiumacetobutylicump262 |