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Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection
The prevalence of HIV-1 is highest in Sub-Saharan Africa. Protective immune responses directed against HIV are complex and involve both cellular and humoral immunity. Based on the recent finding that the best correlate of protection against the first protective prophylactic RV144 vaccine were HIV-sp...
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| Format: | Thesis |
| Language: | English |
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Division of Virology
2017
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| _version_ | 1867613225628991488 |
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| access_status_str | Open Access |
| author | Mbodo, Iyaloo |
| author2 | Passmore, Jo-Ann |
| author_browse | Mbodo, Iyaloo Passmore, Jo-Ann |
| author_facet | Passmore, Jo-Ann Mbodo, Iyaloo |
| author_sort | Mbodo, Iyaloo |
| collection | Thesis |
| description | The prevalence of HIV-1 is highest in Sub-Saharan Africa. Protective immune responses directed against HIV are complex and involve both cellular and humoral immunity. Based on the recent finding that the best correlate of protection against the first protective prophylactic RV144 vaccine were HIV-specific antibody responses, including those mediating natural killer (NK) cell antibody-dependent cellular cytotoxicity (ADCC), there has been considerable interest in measuring alternative roles for HIV-specific binding antibodies. The aim of this MSc dissertation was to optimise and compare two high-throughput flow cytometry based approaches - the GranToxilux and PanToxilux assays - to measure HIV-specific ADCC responses. To do this, NK cells from a panel of healthy HIV-negative individuals were screened for their ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against K562 cells was chosen as the universal NK cell donor for this study. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/24300 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:45.765Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Division of Virology |
| publisherStr | Division of Virology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/24300 Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection Mbodo, Iyaloo Passmore, Jo-Ann Gamieldien, Hoyam Virology The prevalence of HIV-1 is highest in Sub-Saharan Africa. Protective immune responses directed against HIV are complex and involve both cellular and humoral immunity. Based on the recent finding that the best correlate of protection against the first protective prophylactic RV144 vaccine were HIV-specific antibody responses, including those mediating natural killer (NK) cell antibody-dependent cellular cytotoxicity (ADCC), there has been considerable interest in measuring alternative roles for HIV-specific binding antibodies. The aim of this MSc dissertation was to optimise and compare two high-throughput flow cytometry based approaches - the GranToxilux and PanToxilux assays - to measure HIV-specific ADCC responses. To do this, NK cells from a panel of healthy HIV-negative individuals were screened for their ability to directly kill the tumour cell line K562, as a measure of direct NK cell cytotoxicity. The individual with the highest granzyme B and caspase activity against K562 cells was chosen as the universal NK cell donor for this study. 2017-05-16T07:54:35Z 2017-05-16T07:54:35Z 2014 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/24300 eng application/pdf Division of Virology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Virology Mbodo, Iyaloo Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| thesis_degree_str | Master's |
| title | Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| title_full | Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| title_fullStr | Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| title_full_unstemmed | Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| title_short | Comparing high-throughput methods to measure antibody dependent cellular cytotoxicity during HIV infection |
| title_sort | comparing high throughput methods to measure antibody dependent cellular cytotoxicity during hiv infection |
| topic | Virology |
| url | http://hdl.handle.net/11427/24300 |
| work_keys_str_mv | AT mbodoiyaloo comparinghighthroughputmethodstomeasureantibodydependentcellularcytotoxicityduringhivinfection |