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In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma
This thesis records a series of experiments conducted to gain further insight into factors which influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormal...
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| Format: | Thesis |
| Language: | English |
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Division of Pulmonology
2017
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| _version_ | 1867613172385447936 |
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| access_status_str | Open Access |
| author | Potter, Paul Charles |
| author_browse | Potter, Paul Charles |
| author_facet | Potter, Paul Charles |
| author_sort | Potter, Paul Charles |
| collection | Thesis |
| description | This thesis records a series of experiments conducted to gain further insight into factors which influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormality in allergic asthma could be induced, induced by autoantibodies. I established and optimised beta-2 adrenergic receptor ligand binding and functional assays in guinea pig lung membranes and then conducted an original study of beta adrenergic receptor expression in the guinea pig foetal lung. I found that beta adrenergic receptor expression in the foetal lung was dormant for 80% of the gestation period. After day 53 there was a surge in receptor expression which increased beyond term. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26483 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:54.917Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Division of Pulmonology |
| publisherStr | Division of Pulmonology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26483 In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma Potter, Paul Charles Asthma - etiology Ligands Receptors, Adrenergic, beta - genetics Receptors, Adrenergic, beta - immunology This thesis records a series of experiments conducted to gain further insight into factors which influence the expression, ligand binding and functional activity of the beta-2 adrenergic receptor. These studies were prompted by previous reports that the postulated beta-2 adrenergic receptor abnormality in allergic asthma could be induced, induced by autoantibodies. I established and optimised beta-2 adrenergic receptor ligand binding and functional assays in guinea pig lung membranes and then conducted an original study of beta adrenergic receptor expression in the guinea pig foetal lung. I found that beta adrenergic receptor expression in the foetal lung was dormant for 80% of the gestation period. After day 53 there was a surge in receptor expression which increased beyond term. 2017-12-07T07:58:42Z 2017-12-07T07:58:42Z 1991 2017-08-07T10:59:12Z Doctoral Thesis Doctoral MD http://hdl.handle.net/11427/26483 eng application/pdf Division of Pulmonology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Asthma - etiology Ligands Receptors, Adrenergic, beta - genetics Receptors, Adrenergic, beta - immunology Potter, Paul Charles In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| thesis_degree_str | Doctoral |
| title | In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| title_full | In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| title_fullStr | In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| title_full_unstemmed | In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| title_short | In Vitro studies of factors which may influence ligand binding, function, immunogenicity and genetic regulation of the Beta-2 adrenergic receptor in asthma |
| title_sort | in vitro studies of factors which may influence ligand binding function immunogenicity and genetic regulation of the beta 2 adrenergic receptor in asthma |
| topic | Asthma - etiology Ligands Receptors, Adrenergic, beta - genetics Receptors, Adrenergic, beta - immunology |
| url | http://hdl.handle.net/11427/26483 |
| work_keys_str_mv | AT potterpaulcharles invitrostudiesoffactorswhichmayinfluenceligandbindingfunctionimmunogenicityandgeneticregulationofthebeta2adrenergicreceptorinasthma |