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The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
Bibliography: leaves 231-243.
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| Format: | Thesis |
| Language: | English |
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Division of Chemical Pathology
2014
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| _version_ | 1867613330216058880 |
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| access_status_str | Open Access |
| author | Phehane, Vuyisile Ntosi |
| author2 | Mclntosh, David |
| author_browse | Mclntosh, David Phehane, Vuyisile Ntosi |
| author_facet | Mclntosh, David Phehane, Vuyisile Ntosi |
| author_sort | Phehane, Vuyisile Ntosi |
| collection | Thesis |
| description | Bibliography: leaves 231-243. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/2701 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:25.395Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Chemical Pathology |
| publisherStr | Division of Chemical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/2701 The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) Phehane, Vuyisile Ntosi Mclntosh, David Chemical Pathology Bibliography: leaves 231-243. We have expressed and purified human, two forms of P. falciparum, and Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HPRT) in E. coli using the pET expression system. The cDNA encoding the ORF of HPRT was amplified by PCR and transformed into E. coli cells using standard methods. Expression was induced by IPTG and reached about 13% of the total cell protein for all four proteins. The HPRTs were purified by nickel affinity chromatography most of the expressed protein could be isolated from the crude supernatant fraction in a soluble form. Human HPRT was active, with activity levels in the region of 38 umoles GMP min⁻¹ mg⁻¹ at 37 ⁰C, which is comparable to published literature values. 2014-07-28T08:15:16Z 2014-07-28T08:15:16Z 2002 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/2701 eng application/pdf Division of Chemical Pathology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Chemical Pathology Phehane, Vuyisile Ntosi The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| thesis_degree_str | Doctoral |
| title | The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| title_full | The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| title_fullStr | The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| title_full_unstemmed | The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| title_short | The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) |
| title_sort | expression and drug targeting of parasitic hypoxanthine guanine phosphoribosyltransferase hgprt |
| topic | Chemical Pathology |
| url | http://hdl.handle.net/11427/2701 |
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