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A Ca²⁺-activated proteinase in chicken skeletal muscle
A neutral calcium-activated protease of muscle (CAP) has previously been characterised and may play a role in myofibrillar disassembly and turnover. In this study both CAP and endogenous CAP inhibitor from adult and embryonic chicken skeletal muscle have been partially purified by DEAE-cellulose and...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Biochemistry and Structural Biology
2018
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| _version_ | 1867613348029267968 |
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| access_status_str | Open Access |
| author | Smith, Arlene Atkinson |
| author2 | Van der Westhuyzen, Deneys R |
| author_browse | Smith, Arlene Atkinson Van der Westhuyzen, Deneys R |
| author_facet | Van der Westhuyzen, Deneys R Smith, Arlene Atkinson |
| author_sort | Smith, Arlene Atkinson |
| collection | Thesis |
| description | A neutral calcium-activated protease of muscle (CAP) has previously been characterised and may play a role in myofibrillar disassembly and turnover. In this study both CAP and endogenous CAP inhibitor from adult and embryonic chicken skeletal muscle have been partially purified by DEAE-cellulose and Sephadex G-150 chromatography. CAP from embryonic muscle shows similar properties to the corresponding enzyme from adult tissue with respect to calcium dependence (maximum activity at 1.0 rnM Ca²⁺), pH optimum (7.2) and sensitivity to proteinase inhibitors (inhibited by leupeptin and chymostatin). Both embryonic and adult enzymes were found to have molecular weights of 112000 daltons by gel filtration on Sephadex G-150. CAP activity was present in cultured skeletal muscle cells and increased with cellular growth and differentiation (five-fold). The presence of an inhibitor of CAP was demonstrated in cell cultures by ion-exchange chromatography, the levels of which decreased with a simultaneous increase in CAP activity. CAP activity showed an increase in developing muscle from 12-day embryos to 7-week chicks in relation to cellular DNA (3.8- fold), although the extent of this increase did not match the extent of accumulation of myofibrillar proteins. High levels of CAP inhibitor were found in early embryonic muscle and these decreased markedly during development. CAP inhibitor from embryonic tissue was fractionated into 3 species using DEAE-cellulose in contrast to inhibitor from adult tissue which exhibited only two species. The results indicate that the levels of CAP greatly increase at a time when myofibrillar content of muscle is rapidly increasing and, in addition, demonstrate that CAP activity may be controlled to a large extent by the levels of an intracellular inhibitor. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27268 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:42.993Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Medical Biochemistry and Structural Biology |
| publisherStr | Division of Medical Biochemistry and Structural Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27268 A Ca²⁺-activated proteinase in chicken skeletal muscle Smith, Arlene Atkinson Van der Westhuyzen, Deneys R Medical Biochemistry Myofibrils - Physiology Endopeptidases Calcium-binding proteins A neutral calcium-activated protease of muscle (CAP) has previously been characterised and may play a role in myofibrillar disassembly and turnover. In this study both CAP and endogenous CAP inhibitor from adult and embryonic chicken skeletal muscle have been partially purified by DEAE-cellulose and Sephadex G-150 chromatography. CAP from embryonic muscle shows similar properties to the corresponding enzyme from adult tissue with respect to calcium dependence (maximum activity at 1.0 rnM Ca²⁺), pH optimum (7.2) and sensitivity to proteinase inhibitors (inhibited by leupeptin and chymostatin). Both embryonic and adult enzymes were found to have molecular weights of 112000 daltons by gel filtration on Sephadex G-150. CAP activity was present in cultured skeletal muscle cells and increased with cellular growth and differentiation (five-fold). The presence of an inhibitor of CAP was demonstrated in cell cultures by ion-exchange chromatography, the levels of which decreased with a simultaneous increase in CAP activity. CAP activity showed an increase in developing muscle from 12-day embryos to 7-week chicks in relation to cellular DNA (3.8- fold), although the extent of this increase did not match the extent of accumulation of myofibrillar proteins. High levels of CAP inhibitor were found in early embryonic muscle and these decreased markedly during development. CAP inhibitor from embryonic tissue was fractionated into 3 species using DEAE-cellulose in contrast to inhibitor from adult tissue which exhibited only two species. The results indicate that the levels of CAP greatly increase at a time when myofibrillar content of muscle is rapidly increasing and, in addition, demonstrate that CAP activity may be controlled to a large extent by the levels of an intracellular inhibitor. 2018-02-05T12:42:19Z 2018-02-05T12:42:19Z 1981 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/27268 eng application/pdf Division of Medical Biochemistry and Structural Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Biochemistry Myofibrils - Physiology Endopeptidases Calcium-binding proteins Smith, Arlene Atkinson A Ca²⁺-activated proteinase in chicken skeletal muscle |
| thesis_degree_str | Master's |
| title | A Ca²⁺-activated proteinase in chicken skeletal muscle |
| title_full | A Ca²⁺-activated proteinase in chicken skeletal muscle |
| title_fullStr | A Ca²⁺-activated proteinase in chicken skeletal muscle |
| title_full_unstemmed | A Ca²⁺-activated proteinase in chicken skeletal muscle |
| title_short | A Ca²⁺-activated proteinase in chicken skeletal muscle |
| title_sort | ca²⁺ activated proteinase in chicken skeletal muscle |
| topic | Medical Biochemistry Myofibrils - Physiology Endopeptidases Calcium-binding proteins |
| url | http://hdl.handle.net/11427/27268 |
| work_keys_str_mv | AT smitharleneatkinson aca2activatedproteinaseinchickenskeletalmuscle AT smitharleneatkinson ca2activatedproteinaseinchickenskeletalmuscle |