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Brain distribution and release of Cholecystokinin octapeptide
In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of B...
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| Format: | Thesis |
| Language: | English |
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Division of Chemical Pathology
2018
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| _version_ | 1867613217787740160 |
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| access_status_str | Open Access |
| author | Hudson, Anne Mary |
| author2 | Millar, Robert P |
| author_browse | Hudson, Anne Mary Millar, Robert P |
| author_facet | Millar, Robert P Hudson, Anne Mary |
| author_sort | Hudson, Anne Mary |
| collection | Thesis |
| description | In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27500 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:38.580Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Chemical Pathology |
| publisherStr | Division of Chemical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27500 Brain distribution and release of Cholecystokinin octapeptide Hudson, Anne Mary Millar, Robert P Neurophysiology Cholecystokinin In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings. 2018-02-12T08:41:03Z 2018-02-12T08:41:03Z 1980 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/27500 eng application/pdf Division of Chemical Pathology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Neurophysiology Cholecystokinin Hudson, Anne Mary Brain distribution and release of Cholecystokinin octapeptide |
| thesis_degree_str | Master's |
| title | Brain distribution and release of Cholecystokinin octapeptide |
| title_full | Brain distribution and release of Cholecystokinin octapeptide |
| title_fullStr | Brain distribution and release of Cholecystokinin octapeptide |
| title_full_unstemmed | Brain distribution and release of Cholecystokinin octapeptide |
| title_short | Brain distribution and release of Cholecystokinin octapeptide |
| title_sort | brain distribution and release of cholecystokinin octapeptide |
| topic | Neurophysiology Cholecystokinin |
| url | http://hdl.handle.net/11427/27500 |
| work_keys_str_mv | AT hudsonannemary braindistributionandreleaseofcholecystokininoctapeptide |