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Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin
Haliotis midae aquaculture within South Africa remains afflicted by infectious diseases. Understanding of how the abalone’s innate immune response functions is one of the greatest hindrances to assisting with the defence or detection of pathogenic attacks on farms. The multifunctional oxygen transpo...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2020
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| _version_ | 1867613939126239232 |
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| access_status_str | Open Access |
| author | North, Jarid |
| author2 | Coyne, Vernon |
| author_browse | Coyne, Vernon North, Jarid |
| author_facet | Coyne, Vernon North, Jarid |
| author_sort | North, Jarid |
| collection | Thesis |
| description | Haliotis midae aquaculture within South Africa remains afflicted by infectious diseases. Understanding of how the abalone’s innate immune response functions is one of the greatest hindrances to assisting with the defence or detection of pathogenic attacks on farms. The multifunctional oxygen transporter haemocyanin was previously found to be upregulated in response to bacterial infection (Beltran 2015), indicating it may play a role in the defence response of Haliotis midae. The current knowledge regarding haemocyanin’s role in the abalone innate immune response is incomplete. A number of studies have been published that investigated haemocyanin’s potential as a broad spectrum antimicrobial peptide in many arthropod species. There has been only one study conducted in molluscs, which utilised synthetic peptides derived from a haemocyanin consensus sequence. In most organisms the haemocyanin protein is comprised of a string of eight roughly 50 kDa functional units (FU) annotated a-h. The current study determined the nucleotide sequence of the final four functional units on the C-terminal end of H. midae haemocyanin and examined the potential antimicrobial activity of the peptide product. The haliotisin coding sequence identified by Zhuang et al. (2015) was detected in FU-e of the H. midae haemocyanin and subcloned into an Escherichia coli expression vector for recombinant production of the peptide. This peptide showed some activity against Staphylococcus aureus and E. coli, suggesting it may function as an antimicrobial peptide. This study provides the first evidence that an antimicrobial peptide derived from the H. midae haemocyanin could be functioning as a component of the abalone innate immune response. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/31083 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:44:06.707Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/31083 Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin North, Jarid Coyne, Vernon Molecular and Cell Biology Haliotis midae aquaculture within South Africa remains afflicted by infectious diseases. Understanding of how the abalone’s innate immune response functions is one of the greatest hindrances to assisting with the defence or detection of pathogenic attacks on farms. The multifunctional oxygen transporter haemocyanin was previously found to be upregulated in response to bacterial infection (Beltran 2015), indicating it may play a role in the defence response of Haliotis midae. The current knowledge regarding haemocyanin’s role in the abalone innate immune response is incomplete. A number of studies have been published that investigated haemocyanin’s potential as a broad spectrum antimicrobial peptide in many arthropod species. There has been only one study conducted in molluscs, which utilised synthetic peptides derived from a haemocyanin consensus sequence. In most organisms the haemocyanin protein is comprised of a string of eight roughly 50 kDa functional units (FU) annotated a-h. The current study determined the nucleotide sequence of the final four functional units on the C-terminal end of H. midae haemocyanin and examined the potential antimicrobial activity of the peptide product. The haliotisin coding sequence identified by Zhuang et al. (2015) was detected in FU-e of the H. midae haemocyanin and subcloned into an Escherichia coli expression vector for recombinant production of the peptide. This peptide showed some activity against Staphylococcus aureus and E. coli, suggesting it may function as an antimicrobial peptide. This study provides the first evidence that an antimicrobial peptide derived from the H. midae haemocyanin could be functioning as a component of the abalone innate immune response. 2020-02-13T09:57:13Z 2020-02-13T09:57:13Z 2019 2020-02-13T09:57:00Z Master Thesis Masters MSc http://hdl.handle.net/11427/31083 eng application/pdf Department of Molecular and Cell Biology Faculty of Science |
| spellingShingle | Molecular and Cell Biology North, Jarid Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| thesis_degree_str | Master's |
| title | Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| title_full | Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| title_fullStr | Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| title_full_unstemmed | Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| title_short | Identification of a potential antimicrobial peptide derived from Haliotis midae haemocyanin |
| title_sort | identification of a potential antimicrobial peptide derived from haliotis midae haemocyanin |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/31083 |
| work_keys_str_mv | AT northjarid identificationofapotentialantimicrobialpeptidederivedfromhaliotismidaehaemocyanin |