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Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses
The principal aim of this study was to develop a new bed material suitable for gel exclusion chromatography, which would embrace all the desirable characteristics of available materials used for this purpose and if possible improve on some of them. The two most important limitations of existing gel...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Virology
2020
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| _version_ | 1867613610456383488 |
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| access_status_str | Open Access |
| author | Katz, Woolf |
| author_browse | Katz, Woolf |
| author_facet | Katz, Woolf |
| author_sort | Katz, Woolf |
| collection | Thesis |
| description | The principal aim of this study was to develop a new bed material suitable for gel exclusion chromatography, which would embrace all the desirable characteristics of
available materials used for this purpose and if possible improve on some of them. The two most important limitations of existing gel media concern their exclusion limits and rigidity. Agar and agarose have been found satisfactory for the separation of larger molecules including some viruses, but difficulties may arise at low gel concentrations due to the low mechanical strength of the granules. The dextran and polyacrylamide gels have in general a rigidity equal to that of agar and agarose, but cannot be prepared with such large pore sizes and are therefore limited to the separation of smaller molecules. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/32028 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:38:53.263Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Division of Medical Virology |
| publisherStr | Division of Medical Virology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/32028 Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses Katz, Woolf Chromatography The principal aim of this study was to develop a new bed material suitable for gel exclusion chromatography, which would embrace all the desirable characteristics of available materials used for this purpose and if possible improve on some of them. The two most important limitations of existing gel media concern their exclusion limits and rigidity. Agar and agarose have been found satisfactory for the separation of larger molecules including some viruses, but difficulties may arise at low gel concentrations due to the low mechanical strength of the granules. The dextran and polyacrylamide gels have in general a rigidity equal to that of agar and agarose, but cannot be prepared with such large pore sizes and are therefore limited to the separation of smaller molecules. 2020-06-02T11:34:41Z 2020-06-02T11:34:41Z 1970 2020-04-07T20:15:48Z Doctoral Thesis Doctoral https://hdl.handle.net/11427/32028 eng application/pdf Division of Medical Virology Faculty of Health Sciences |
| spellingShingle | Chromatography Katz, Woolf Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| thesis_degree_str | Doctoral |
| title | Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| title_full | Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| title_fullStr | Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| title_full_unstemmed | Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| title_short | Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| title_sort | tanned gelatin some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses |
| topic | Chromatography |
| url | https://hdl.handle.net/11427/32028 |
| work_keys_str_mv | AT katzwoolf tannedgelatinsomebiophysicalandbiochemicalpropertiesofanewgelexclusionagentanditsapplicationtothechromatographyofproteinsandviruses |