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Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
Diminishing oil reserves are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fue...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2024
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| _version_ | 1867614308237574144 |
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| access_status_str | Open Access |
| author | Robinson, Evan J |
| author2 | Lindsey, George |
| author_browse | Lindsey, George Robinson, Evan J |
| author_facet | Lindsey, George Robinson, Evan J |
| author_sort | Robinson, Evan J |
| collection | Thesis |
| description | Diminishing oil reserves are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fuel ethanol. In this study, three xylose isomerase genes originating from Haemophilus influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting strains were analysed for protein production, growth differences and xylose isomerisation but no introduced characteristics were detected. RT-PCR suggested transcription occurred for all the genes tested but no recombinant protein nor any xylose isomerase activity was detected. An in silico bioinformatic analysis showed a putative inhibitory stem-loop structure in the mRNA containing the B. thetaiotaomicron gene which may have reduced translation. Otherwise non-functional folding or undetectable activity were concluded as the results of the expression for all genes. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/39405 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:49:58.719Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/39405 Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae Robinson, Evan J Lindsey, George Molecular and Cell Biology Diminishing oil reserves are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fuel ethanol. In this study, three xylose isomerase genes originating from Haemophilus influenzae Rd K20, Arabidopsis thaliana and Bacteroides thetaiotaomicron VPI-5482 were expressed in S. cerevisiae to produce a strain capable of metabolising D-xylose (a biomass constituent). Resulting strains were analysed for protein production, growth differences and xylose isomerisation but no introduced characteristics were detected. RT-PCR suggested transcription occurred for all the genes tested but no recombinant protein nor any xylose isomerase activity was detected. An in silico bioinformatic analysis showed a putative inhibitory stem-loop structure in the mRNA containing the B. thetaiotaomicron gene which may have reduced translation. Otherwise non-functional folding or undetectable activity were concluded as the results of the expression for all genes. 2024-04-17T14:02:18Z 2024-04-17T14:02:18Z 2008 2024-04-17T13:29:12Z Thesis / Dissertation Masters MSc http://hdl.handle.net/11427/39405 eng application/pdf Department of Molecular and Cell Biology Faculty of Science |
| spellingShingle | Molecular and Cell Biology Robinson, Evan J Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| thesis_degree_str | Master's |
| title | Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| title_full | Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| title_fullStr | Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| title_full_unstemmed | Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| title_short | Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae |
| title_sort | evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in saccharomyces cerevisiae |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/39405 |
| work_keys_str_mv | AT robinsonevanj evaluationofthreeheterologousxyloseisomerasegenesforthefermentationofplantbiomasstobioethanolinsaccharomycescerevisiae |