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The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants
The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blo...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2024
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| Summary: | The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. |
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