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The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia
Includes abstract.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Clinical Haematology
2014
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| _version_ | 1867613284780212224 |
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| access_status_str | Open Access |
| author | Du Pisani, Louis Almero |
| author2 | Shires, Karen |
| author_browse | Du Pisani, Louis Almero Shires, Karen |
| author_facet | Shires, Karen Du Pisani, Louis Almero |
| author_sort | Du Pisani, Louis Almero |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/6558 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:41.762Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Clinical Haematology |
| publisherStr | Division of Clinical Haematology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/6558 The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia Du Pisani, Louis Almero Shires, Karen Includes abstract. Includes bibliographical references. Nucleophosmin (NPM1) is an acidic, nucleo-cytoplasmic, shuttling protein with predominant nucleolar localisation that plays multiple roles in cell growth and proliferation. Deletion insertion mutations of NPM1 (NPM1 DIM) seem to disrupt it normal physiologic role as a molecular chaperone, which likely leads to its oncogenic potential.NPM1 if present alone (not associated with FLT3 internal tandem duplications (FLT3-ITD)) is associated with significantly better overall survival and disease free survival in AML and has been entered as a provisional category in the World Health Organisation (2008) classification of Acute Myeloid Leukaemia with recurrent genetic abnormalities. Current methodology uses reverse transcriptase polymerase chain reaction (RT-PCR) and genomic deoxyribonucleic acid (DNA) PCR techniques to detect NPM1 DIM. Although these methods are robust and relatively easy to perform they can be expensive, labour intensive and not universally available. Six major variants of NPM1 DIM (Types A-F) have been described all leading to frame shift. All six types share the same last five amino acids in the C-terminal.The aim of this study was to develop a robust flow cytometry methodology that could be used in the routine assessment of AML samples to determine the mutational state of NPM, using a commercially available polyclonal antibody against the mutated NPM1. 2014-08-15T14:04:37Z 2014-08-15T14:04:37Z 2014 Master Thesis Masters MMed http://hdl.handle.net/11427/6558 eng application/pdf Division of Clinical Haematology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Du Pisani, Louis Almero The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| thesis_degree_str | Master's |
| title | The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| title_full | The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| title_fullStr | The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| title_full_unstemmed | The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| title_short | The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia |
| title_sort | development of a flow cytometric method to detect the presence of mutated nucleophosmin in acute myeloid leukaemia |
| url | http://hdl.handle.net/11427/6558 |
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