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Metabolomics of desiccation tolerance in Xerophyta humilis
Includes bibliographical references
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613230115848192 |
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| access_status_str | Open Access |
| author | Dace, Halford |
| author2 | Farrant, Jill M |
| author_browse | Dace, Halford Farrant, Jill M |
| author_facet | Farrant, Jill M Dace, Halford |
| author_sort | Dace, Halford |
| collection | Thesis |
| description | Includes bibliographical references |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9111 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:50.328Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9111 Metabolomics of desiccation tolerance in Xerophyta humilis Dace, Halford Farrant, Jill M Rafudeen, M S Includes bibliographical references Resurrection plants are unique in the ability to survive near complete water loss in vegetative tissues without loss of viability. In order to do so, they employ multifaceted strategies which include structural adaptations, antioxidant and photoprotective mechanisms, and the accumulation of proteins and metabolites that stabilise macromolecules. A full understanding of the phenomenon of vegetative desiccation tolerance will require a systems view of these adaptations at the levels of the genome, the control of gene expression, and the control of metabolic pathways. This dissertation reports a high-throughput metabolomic analysis of the changes that occur in vegetative tissues of resurrection plant Xerophyta humilis during dehydration. A combination of chromatography, mass spectrometry and nuclear magnetic resonance revealed numerous primary and secondary metabolites in the plant. Multivariate statistics identified a subset of metabolites that were significantly up- or down-regulated in response to water deficit stress. These metabolites both confirmed existing observations about the metabolic response of X. humilis to drying and revealed compounds not previously known to be associated with this response. Desiccation-associated metabolites were mapped onto known biochemical pathways, to generate hypotheses concerning possible regulatory schemes in the stress response, inviting deeper investigation in future. 2014-11-05T03:46:58Z 2014-11-05T03:46:58Z 2014 Master Thesis Masters MSc http://hdl.handle.net/11427/9111 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Dace, Halford Metabolomics of desiccation tolerance in Xerophyta humilis |
| thesis_degree_str | Master's |
| title | Metabolomics of desiccation tolerance in Xerophyta humilis |
| title_full | Metabolomics of desiccation tolerance in Xerophyta humilis |
| title_fullStr | Metabolomics of desiccation tolerance in Xerophyta humilis |
| title_full_unstemmed | Metabolomics of desiccation tolerance in Xerophyta humilis |
| title_short | Metabolomics of desiccation tolerance in Xerophyta humilis |
| title_sort | metabolomics of desiccation tolerance in xerophyta humilis |
| url | http://hdl.handle.net/11427/9111 |
| work_keys_str_mv | AT dacehalford metabolomicsofdesiccationtoleranceinxerophytahumilis |