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Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Chemistry
2014
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| _version_ | 1867613251214245888 |
|---|---|
| access_status_str | Open Access |
| author | Singh, Manooj |
| author2 | Jackson, Graham Ellis |
| author_browse | Jackson, Graham Ellis Singh, Manooj |
| author_facet | Jackson, Graham Ellis Singh, Manooj |
| author_sort | Singh, Manooj |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9604 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:10.259Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Chemistry |
| publisherStr | Department of Chemistry |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9604 Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions Singh, Manooj Jackson, Graham Ellis Chemistry Includes bibliographical references. Acute and Chronic inflammations in Rheumatoid Arthritis are characterized by, among other features, changes in the metabolism of copper. Previous studies have shown that administration of exogenous copper, and the in vivo manipulation of endogenous copper may provide new ways of coping with the problem of anti-inflammatory/anti-arthritic therapies. Computer models of blood plasma have attempted to rationalize the use of copper complexes in this regard. This thesis describes the design and commissioning of an ultrafiltration apparatus to study metal interactions with blood proteins. The results are compared with equilibrium dialysis. The systems studied using this technique includes, the binding of Cu(ll) to HSA and BSA, ternary Cu(ll)-HSA-amino acid systems, as well as competitive studies of the Cu(ll)-HSA system with Mg(ll), Ca(ll) and Zn(ll). The ultrafiltrate was analysed using HPLC and atomic absorption spectroscopy. Protein preparation was found to be important and the different methods used for protein purification were compared using gel electrophoresis and atomic absorption. Binding studies of chelating agents as well as several pharmaceuticals in human blood serum were carried out and compared with computer modelling studies. 2014-11-14T03:57:45Z 2014-11-14T03:57:45Z 1998 Master Thesis Masters MSc http://hdl.handle.net/11427/9604 eng application/pdf Department of Chemistry Faculty of Science University of Cape Town |
| spellingShingle | Chemistry Singh, Manooj Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| thesis_degree_str | Master's |
| title | Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| title_full | Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| title_fullStr | Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| title_full_unstemmed | Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| title_short | Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions |
| title_sort | copper and rheumatoid arthritis an in vitro study of metal protein interactions |
| topic | Chemistry |
| url | http://hdl.handle.net/11427/9604 |
| work_keys_str_mv | AT singhmanooj copperandrheumatoidarthritisaninvitrostudyofmetalproteininteractions |