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Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria
Dissertation (MSc)--University of Pretoria, 2013.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613682938150912 |
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| access_status_str | Open Access |
| author2 | Van Vuuren, Moritz |
| author_browse | Van Vuuren, Moritz |
| author_facet | Van Vuuren, Moritz |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/40708 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:01.664Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/40708 Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria Van Vuuren, Moritz samantipx@yahoo.com Quan, Melvyn Shamaki, D. Mantip, Samuel Elias Lashat Peste des petits ruminants virus (PPRV) Paramyxoviridae Genus morbillivirus UCTD Dissertation (MSc)--University of Pretoria, 2013. Peste des petits ruminants virus (PPRV) belongs to the family Paramyxoviridae and genus morbillivirus. It is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militate against the production of sheep and goats in Nigeria. It is a notifiable disease according to the World Organization for Animal Health (Office International des Epizooties). In this study, a molecular analysis of PPRV from sheep and goats from recent outbreaks across the different regions of Nigeria was carried out. The aim was to describe the viral strains and the movement of the virus within the country compared to other endemic areas of the world. This was carried out through tissue and swab samples collected from sheep and goats in various agro-ecological zones of Nigeria.The evolution and relationship of earlier PPRV strains/isolates and those circulating and causing recent outbreaks was determined by sequencing of the nucleoprotein (N)-gene. Twenty tissue and swab samples from apparently healthy and sick sheep and goats were collected randomly from each of three states of each of the six agro-ecological zones visited. A total of 360 samples were collected. A total of 35 samples of 360 (9.7 %) tested positive by RT-PCR, of which 25 were from oculo-nasal swabs and 10 were from tissue samples (Table 4.2). Phylogenetic analysis was carried out using the N-gene sequences of the PPRV amplicons. Alignment of the sequences and related sequences from GenBank and neighbor-joining phylogenetic analysis using PAUP identified four different lineages, i.e. lineages I, II, III and IV. Interestingly, the Nigerian strains described in this study grouped in two separate major lineages i.e. lineages II and IV. Strains from Sokoto, Oyo, Plateau and Ondo states grouped according to the historical distribution of PPRV together with the Nigerian 75/1 strain of lineage II, while other strains from Sokoto, Oyo, Plateau, Akwa-Ibom, Adamawa, Kaduna, Lagos, Bauchi, Niger and Kano states grouped together with the East-African and Asian strains of lineage IV. This finding suggests that both lineages II and IV strains of PPRVs are circulating presently in Nigeria, contrary to an earlier publication which indicated that only strains of lineage II were circulating in the country (Shamaki, 2002). gm2014 Veterinary Tropical Diseases unrestricted 2014-07-11T06:22:21Z 2014-07-11T06:22:21Z 2014-04-11 2013 Dissertation Mantip, SEL 2013, Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/40708> E14/4/261/gm http://hdl.handle.net/2263/40708 en © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Peste des petits ruminants virus (PPRV) Paramyxoviridae Genus morbillivirus UCTD Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title | Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title_full | Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title_fullStr | Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title_full_unstemmed | Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title_short | Molecular characterisation of peste des petits ruminants viruses in sheep and goats from Nigeria |
| title_sort | molecular characterisation of peste des petits ruminants viruses in sheep and goats from nigeria |
| topic | Peste des petits ruminants virus (PPRV) Paramyxoviridae Genus morbillivirus UCTD |
| url | http://hdl.handle.net/2263/40708 |