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Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites
Dissertation (MSc (Biochemistry))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613561343180800 |
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| access_status_str | Open Access |
| author2 | Niemand, Jandeli |
| author_browse | Niemand, Jandeli |
| author_facet | Niemand, Jandeli |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biochemistry))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94708 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:05.905Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94708 Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites Niemand, Jandeli u18001719@tuks.co.za Birkholtz, Lyn-Marie Maré, Marché UCTD Plasmodium Nutrient acquisition Amino acid transporters Conditional knockdown Gene disruption Genetic manipulation Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-04: Quality education Natural and agricultural sciences theses SDG-04 Dissertation (MSc (Biochemistry))--University of Pretoria, 2024. Although malaria is a curable disease, the causative agent, the Plasmodium falciparum parasite, continuously develops antimalarial resistance, making current chemotherapeutics ineffective. The parasites are amino acid auxotrophs, and amino acids are mostly obtained from haemoglobin digestion, with additional amino acids, such as isoleucine and methionine, obtained from the extracellular environment. The uptake of these extracellular amino acids requires transport across multiple membranes surrounding the parasite. Two putative amino acid transporters from the neurotransmitter:sodium symporter family are constitutively expressed in P. falciparum asexual parasites and gametocytes, and we hypothesize that these are essential for asexual proliferation and sexual differentiation in P. falciparum parasites. Here, our objective was to develop genetically modified parasite lines to investigate the essentiality of these two putative amino acid transporters. NRF Biochemistry MSc (Biochemistry) Unrestricted Faculty of Natural and Agricultural Sciences 2024-02-19T09:24:26Z 2024-02-19T09:24:26Z 2024-04-01 2024-02-15 Dissertation * A2024 http://hdl.handle.net/2263/94708 10.25403/UPresearchdata.25211261 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Plasmodium Nutrient acquisition Amino acid transporters Conditional knockdown Gene disruption Genetic manipulation Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-04: Quality education Natural and agricultural sciences theses SDG-04 Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title | Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title_full | Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title_fullStr | Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title_full_unstemmed | Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title_short | Towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| title_sort | towards genetic interrogation of putative amino acid transporters in plasmodium falciparum parasites |
| topic | UCTD Plasmodium Nutrient acquisition Amino acid transporters Conditional knockdown Gene disruption Genetic manipulation Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-04: Quality education Natural and agricultural sciences theses SDG-04 |
| url | http://hdl.handle.net/2263/94708 |